Biolight Reagent & Consumables

BioLight reagent is manufactured using the marine bacterium Aliivibrio fischeri* (NRRL B-11177). This strain of bacteria meets the ISO 11348-3 and has been used for over 30 years to measure toxicity in water, sediments, soil . It is sensitive to over 3,600 chemical compounds.

biolight reagnent image 3

REAGENT

Aqua Science, BioLight Reagent comes in both Single test (for field protocols) and Multi Test (for laboratory protocols).  BioLight Reagent has sensitivity to up to 3600 contaminants simultaneously.  The bacterium is from a marine source and has had many names over the years.  It was originally Photobacterium phosphoreum, then Vibrio Fischeri and currently Aliivibrio Fischeri (NRRL B-11177)*. One vial of reagent (1 mL) contains up to one hundred million test organisms.  Shelf life is 3 years from the date of manufacture, stored at minus (-) 20ºC to (-) 25ºC.  Once the reagent is reconstituted, it should be kept in the cooled read well and can be used for 4 hours to run samples

  • BIO2006—BioLight Multi test
  • BIO2007—BioLight Single test

Biolight Recon Solution

BioLight Recon – Recon is made from ultra-pure water that is prepared to meet requirements of reconstituting the reagent.  It is good for 3 years from the date of manufacture and does not require refrigeration.

  • BIO2006—BioLight Multi test
  • BIO2007—BioLight Single test
  • BIO2015A—Disposable Glass Cuvettes (200)
  • BIO2016A—Disposable Glass Cuvettes (1000)
  • RAW1035—10–100 uL Pipet Tips
  • RAW1036—100–1000 uL Pipet Tips
  • BIO2010—Extended Length Tips for Solid Phase
  • BIO2011—Solid Phase Filter Column and Tubes
biolight recon
biolight diluent

Biolight Diluent

BioLight Diluent – Diluent is a 2% sodium chloride solution used to dilute both the samples and the reagent.  Due to the reagent being a marine bacterium, the 2% sodium chloride gives osmotic protection.  It is good for 3 years from the date of manufacture and does not require refrigeration.

  • BIO2001—BioLight Recon (50 mL)
  • BIO2002—BioLight Diluent (1 L)
  • BIO2003—BioLight Solid Phase Diluent
  • BIO2004—BioLight Salinity Adjustment (50 mL)
  • BIO2005—BioLight Recon (1 L)

BioLight Salinity Adjustment

The reagent requires osmotic adjustment which is done using a solution of 22% sodium chloride solution, adjusting the solution to ~ 2% sodium chloride. 

  • BIO2001—BioLight Recon (50 mL)
  • BIO2002—BioLight Diluent (1 L)
  • BIO2003—BioLight Solid Phase Diluent
  • BIO2004—BioLight Salinity Adjustment (50 mL)

 

biolight salinity adjusting

CONSUMABLES

cuvettes in the lab

Cuvettes

Cuvettes– Controls, samples and reagent are pipetted into the cuvettes during testing.  Cuvettes are not reusable and should not be washed and dried for later tests.

Pipets and Tips

Pipets and Tips– Pipets and tips will be required to transfer small amounts of liquid.  The most common and recommended pipets are: 10 – 100 µL adjustable volume pipettor 1 – 10 mL adjustable volume pipettor and a repeat pipettor.

  • BIO2015A—Disposable Glass Cuvettes (200)
  • BIO2016A—Disposable Glass Cuvettes (1000)
  • RAW1035—10–100 uL Pipet Tips
  • RAW1036—100–1000 uL Pipet Tips
  • BIO2010—Extended Length Tips for Solid Phase
  • BIO2011—Solid Phase Filter Column and Tubes

 

cuvettes in a box

Explanation of use:

BioLight Reagent is made of bioluminescent bacteria used for acute toxicity testing. It is freeze dried for extended life then stored frozen at -20 to -25 C 

 and is reconstituted before use with Biolight Recon Solution.

 A single vial of reagent contains approximately one hundred million cells. The sample is salinity adjusted with Biolight Adjusting Solution to 

ensure a saltwater atmosphere due to Aliivibrio fischeri being a marine bacterium.

BioLight Diluent is used for required for the protocol. The biomluminescent reagent elicits the brightest light levels following reconstitions.

These light levels are measured prior to sample exposure and over time after exposure to a simple , using a luminometer measures

the changes in the light to indicates the levels of toxicity.

BioLight Reagents are produced and tested under strict Quality Control and will include a Quality Certificate as per requirements of

the International Standard Organization,ISO 11248-3  

 

* rRNA comparison led to the reclassification from genus Vibrio fischeri to the newly created Aliivibrio fischeri in 2007. NRRL B-11177