BioLight Toxy

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The Aqua Science BioLight Toxy along with the BioLight Reagents and Consumables brings rapid toxicity testing to the 21st Century.


It offers the most up to date, dual option instrument available for bioluminescent analysis in the market. This is a result of matching a team of experts in ecotoxicity to fulfill the needs of toxicity users around the world.


This state-of-the art luminometer brings together the capabilities of doing portable testing in the field as well as bench top testing in the lab. This all-in-one unit is designed to be used in conjunction with the BioLight Multi and Single Reagent as well as BioLight salinity adjusting solution, BioLight diluent and BioLight Recon. The BioLight Toxy instrument is used to read the differences in light from time zero and after exposure to a sample which could be toxic.

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BioLight Toxy PORTABLE

When using the BioLight Toxy in the field, two standard protocols are set up in the onboard software, the Screening Test and the Basic Test. This testing requires the BioLight Single Test Reagent, BioLight Diluent, BioLight Salinity Adjusting Solution and disposable glass cuvettes.


The BioLight Toxy field portable mode does not include temperature control, so it is important to keep the reagent frozen until use. Each vial of Single reagent will do up to 3 tests. Typically, this will be a negative and positive control and 1 sample. To start, sample tubes are set up in a cuvette rack based on the number to be analyzed. Following the protocol in the instrument, the reagent will be prepared and will require a 15-minute wait time to equilibrate. Step by step instructions will be provided in the software protocol for sample prep and reading. The BioLight Toxy will read the Single reagent at time zero to have a baseline to compare the readings after sample exposure. The instrument can be set for custom read times, however the typical field read time is 5 minutes.


This test will provide results in 20 minutes total. The Toxy can be set to read again at 15 minutes if required to see if there is a significant difference in the light loss. Different chemicals often have effects on the light change at different times. Light loss may be present at 5 minutes but may continue to decrease at 15 minutes. The 15-minute reading may be more reliable.

BioLight Toxy BENCH TOP

The BioLight Toxy bench top instrument includes a cooled read well and cooled reagent well along with a separate cooling block to ensure all samples are kept at the appropriate temperatures during testing. There is also the option to add an additional cooling block to double the number of samples that can be tested.

Additionally required to perform the analysis are the BioLight Multi Reagent, BioLight Diluent, BioLight Recon, BioLight Salinity Adjusting and disposable glass cuvettes.


When using the BioLight Toxy bench top, there will be multiple protocols available to choose in the onboard software. The software is easy to use and provides all results calculations that can be printed or uploaded to a computer as well as saved in the software. Choosing a protocol will be based on the water type, expected toxicity level and results of the baseline data if available. Most protocols provide results in approximately 45 minutes, including the 15 minutes for the BioLight Multi reagent to equilibrate after reconstitution.

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  • 16 GB Storage
  • USB/Wifi (Optional)
  • SCADA Connectivity
  • Read time <7 seconds


  • EC50 Test – 2%, 45% or 81.9% – Testing that provides a calculated EC50 value and measure relative toxicity.
    • 2% EC50 Test – samples expected to have a high level of toxicity. Typically, this would be for wastewater influent or pure compounds.
    • 45% EC50 Test – samples of unknown toxicity. This EC50 test measures relative toxicity and is the most adaptable. It is a good option for septage, influent wastewater and water from a treatment digester.
    • 81.9% EC50 – samples expected to have low to medium toxicity. Primarily used for drinking water, wastewater effluent, pore water and stormwater. The expected EC50 level for this test should be between 30% to 50%.
  • % Effect Test – 2%, 45% or 81.9% -Testing using a single concentration. This test will not determine relative toxicity or provide a dose response curve.
    • 2% Effect Test – Used for samples with high toxicity. Wastewater influent, septage, industrial trucked waste.
    • 45% Effect Test – Used for samples of medium toxicity.
    • 81.9% Effect Test – Used with samples of low toxicity such as drinking water.
  • Comparison Test – Used to compare the relative acute toxicity of an undetermined sample with a control sample. A control sample could be a sample of a determined toxicity. This is the best test for the analysis of low toxicity samples if the EC50 values can’t be achieved using the EC50 test.
  • ISO – Complies with ISO 11348-3. The ISO Standard states that the method is applicable for wastewater, fresh water – both surface and ground, sea and also brackish water, elutes of sediments, pore water – both aqueous extracts and leachates.
  • WET (Whole Effluent Toxicity) Test – This is designed for samples of treated effluent wastewater. It is not an EPA approved method.
  • Solids Test – Used for soils, sediments and other solid samples. The test uses solid samples which are serial diluted with 9.9% being the highest concentration. It can be used for freshwater, marine and estuarine samples with the appropriate diluent. It allows for the detection of toxicity due to the insoluble solids that are not in solution by allowing the test organisms to come in direct contact with the solid sample in an aqueous suspension.